Large-Fragment Deletions Induced by Cas9 Cleavage while Not in the BEs System
نویسندگان
چکیده
منابع مشابه
Large-scale genomic deletions mediated by CRISPR/Cas9 system
Owing to its simplicity of manipulation and high efficiency, the CRISPR/Cas9 technology has already been achieved as a robust genome engineering tool for generating gene mutations, multiple-gene knockout and knock-in in various species. However, tissues and genomic mosaicism have been widely reported in the gentically modified animals generated from zygotes that were coinjected with Cas9 mRNA a...
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BACKGROUND The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potential off-target DNA cleavage from incorrect guide by the 20 nt spacer. RESULT...
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ZFN, TALENs and CRISPR/Cas9 system have been used to generate point mutations and large fragment deletions and insertions in genomic modifications. CRISPR/Cas9 system is the most flexible and fast developing technology that has been extensively used to make mutations in all kinds of organisms. However, the most mutations reported up to date are small insertions and deletions. In this report, CR...
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The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for targeted gene editing in various organisms, including plants, where Cas9/sgRNA-mediated small deletions/insertions at single cleavage sites have been reported in transient and stable transformations, although genetic transmission of edits has been reported only in Arabidopsis and rice. Large chromosomal excision betw...
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Targeted genome editing with the CRISPR/Cas9 system has been used extensively for the selective mutation of plant genes. Here we used CRISPR/Cas9 to disrupt the putative barley (Hordeum vulgare cv. "Golden Promise") endo-N-acetyl-β-D-glucosaminidase (ENGase) gene. Five single guide RNAs (sgRNAs) were designed for different target sites in the upstream part of the ENGase coding region. Targeted ...
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ژورنال
عنوان ژورنال: Molecular Therapy - Nucleic Acids
سال: 2020
ISSN: 2162-2531
DOI: 10.1016/j.omtn.2020.06.019